.. image:: ../graphics/Northwestern_purple_RGB.png
   :width: 30%
   :align: right
   :alt: nulogo
   :target: https://amaral.northwestern.edu/

.. _bleedthrough_docs:


Bleedthrough Module
===================

``flyqma.bleedthrough`` provides methods for correcting for spectral bleedthrough between fluorescent reporter channels.


Background Extraction
---------------------

Tools for extracting the background pixels from a segmented image.

.. automodule:: flyqma.bleedthrough.background
   :members:


Resampling
----------

Tools for resampling a set of pixel intensities.

.. automodule:: flyqma.bleedthrough.resampling
   :members:


Bleedthrough Coefficient Characterization
-----------------------------------------

Tools for characterizing bleedthrough coefficients.

.. automodule:: flyqma.bleedthrough.models
   :members:


Bleedthrough Correction
-----------------------

Tools for correcting measurements subject to bleedthrough.

.. automodule:: flyqma.bleedthrough.correction
   :members:


Visualization
-------------

Tools for visualizing the bleedthrough correction procedure.

.. automodule:: flyqma.bleedthrough.visualization
   :members: